2015 PSRM 10th Biennial Meeting
Won Yun Choi , Bo Yeun Kim, Yu Yeon Lee, Dong Hyuk Shin1, Eun A Park, Eun Young Shin, Da Hyin Chung, You Shin Kim, Woo Sik Lee, Tae Ki Yoon, Dong Ryul Lee.
Objective: Macrophage colony-stimulating factor (M-CSF) and Macrophage colony-stimulating factor Receptor (M-CSFR) has been localized in a variety of tissues and shown to influence proliferation and differentiation of numerous cell types. In contrast to granulocyte-macrophage-CSF (GM-CSF), M-CSF is an essential in regulating trophic macrophages during development. The purpose of the study is to analyze the effect of M-CSF and M-CSFR on human embryonic development.
Design: Expression of MCSF and MCSFR mRNAs were detected in granulose cells (GCs). Also, embryonic development were compared when various culture media supplemented with/-out rhM-CSF were applied.
Materials and Methods: GCs were collected from follicular fluids of patients undergone controlled ovarian hyperstimulation. In GCs, expression levels of M-CSF and M-CSFR genes were measured by real time PCR and immunohistochemistry and their results were compared with clinical outcome. In addition, the effects of rhM-CSF on the embryonic development were analyzed in three culture media system with surplus human embryos. We analyzed the time of blastocyst formation(tBF) by Embryoscope® .
Results: The M-CSF and M-CSFR were detected in GCs of human ovarian follicle and embryos through immunohistochemistry, and their mRNAs were highly expressed in GCs derived from pregnant patients compared to those from non-pregnant women. Also, M-CSFR was highly expressed in the blastocyst in comparison with arrested embryos. When surplus embryos were cultured in three media supplemented with rhM-CSF, the rate of blastocyst formation in all groups was highly increased compared to those of non-treated group (59.3% vs. 33.8%, p<0.05) .
Surplus thawed embryos which has formed into blastocyst , supplemented with rhM-CSF group had faster timings compared to non- treated group( 105.9±3.8h vs. 118.3±4.9h, Blastocyst formation range at fresh cycle <96.2h).