제   목 Timing of cell division in human cleavage-stage embryos could predict embryonic potentials for implantation and pregnancy outcomes
등록일 2015/12/18 조회수 474 첨부파일
2015 PSRM 10th Biennial Meeting
2015 KSRM 69th Annual Meeting

Park EA, Choi WY, Jeon YM, Joe EB, Bang SY, Kim ES, Lee MR, Kang KY,  Baek JI, Kwak IP, Yoon TK, Lee DR

Introduction
Currently single embryo transfer is progressively promoted in clinical center for assisted reproductive center as an efficient strategy of avoiding multiple pregnancy. Consequently, the demand to improve implantation rates is important. An essential step for successful In Vitro Fertilization (IVF) outcome is the selection of high-quality embryos for transfer to the woman. The traditionally used method for choosing the best embryos is allowed grading systems based on morphology of embryos, and it is not predictive enough to allow routine single embryo transfer.
EmbryoScope time-lapse system is an emerging continuous and non-invasive embryo observation technique without the need to remove the embryo from optimal culturing conditions. Also this monitoring system enables a detailed evaluation of morphology and morphokinetic parameters. It is used to help select embryos with lower risk of defects and greater potential of implantation and is considered novel clinical opportunities for continuous monitoring of embryos recently.

Objective
Using an EmbryoScope time-lapse system, we have investigated whether morphokinetic parameters of embryos could predict  implantation potentials.

Material & method
Two embryos were selected and transferred to each patient whose implantation rates were then analyzed.

Summary & result
  Many morphokinetic parameters in group A were significantly different  from group C.
  Duration from t3 to t4 (t4-t3) in group A was significantly shorter than that of group C  
    (1.4±4.2 vs 7.6±11.3, p<0.05).
  Timing of cell division in group A is faster than that of group C.
  For analyses between groups B and C, 4 parameters  (2PN, 2PNF, t2, and t3) showed        
    statistical significance.

Conclusion
Our results suggest that time-lapse monitoring for morphokinetic parameters can be used to select embryos with higher implantation potential.